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===User Reviews===
 
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'''UCBerkeley 2012 Team:''' To assay their potential use in our project, we characterized the strength of several registry yeast promoters. We wanted to compare their consistency of expression and relative fluorescence against our lab's strongest promoter, pTDH3. The promoters we used in our assay: pSTE5 (weak), pCYC1 (medium),pADH1 (strong), and  pTDH3 (strongest). <br>
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Experimental Design: We designed our promoters to express a fluorescent protein so that we could quickly measure bulk fluorescence via TECAN. To roughly test if downstream sequence affects expression, we cloned the promoter in front of two different fluorescent proteins, yellow fluorescent Venus and red fluorescent mKate. The device was cloned on a backbone with Leu2 marker and Cen6 origin of replication.
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[[Image:UCBerkeley12_promoter_characterization.png |thumb|none|610px|Promoter Characterization Data. Error bars are +/- one standard deviation. There was very little YFP error.]]
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Revision as of 06:00, 3 October 2012

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Applications of BBa_I766556

User Reviews

UCBerkeley 2012 Team: To assay their potential use in our project, we characterized the strength of several registry yeast promoters. We wanted to compare their consistency of expression and relative fluorescence against our lab's strongest promoter, pTDH3. The promoters we used in our assay: pSTE5 (weak), pCYC1 (medium),pADH1 (strong), and pTDH3 (strongest).

Experimental Design: We designed our promoters to express a fluorescent protein so that we could quickly measure bulk fluorescence via TECAN. To roughly test if downstream sequence affects expression, we cloned the promoter in front of two different fluorescent proteins, yellow fluorescent Venus and red fluorescent mKate. The device was cloned on a backbone with Leu2 marker and Cen6 origin of replication.


Promoter Characterization Data. Error bars are +/- one standard deviation. There was very little YFP error.



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