Difference between revisions of "Part:BBa I752000"
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The riboswitch changes conformation depending on the presence of theophylline. In the absence of theophylline, the switch prevents the binding of the mRNA to the ribosome whilst in the presence of said chemical, the conformation changes and the mRNA behind the switch can then be expressed. | The riboswitch changes conformation depending on the presence of theophylline. In the absence of theophylline, the switch prevents the binding of the mRNA to the ribosome whilst in the presence of said chemical, the conformation changes and the mRNA behind the switch can then be expressed. | ||
| − | [[Part:BBa_K411001]] is an improvement of this part. We used the [[Part:BBa_K411003]] to test the construct. [http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch#Results Experimental results] from iGEM10's NYMU-Taipei team confirm that the part works and has an increased rate of | + | [[Part:BBa_K411001]] is an improvement of this part. We used the [[Part:BBa_K411003]] to test the construct. [http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch#Results Experimental results] from iGEM10's NYMU-Taipei team confirm that the part works and has an increased rate of the translation of the downstream protein coding region when the concentration of Theophylline increases. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:19, 28 October 2010
Riboswitch(theophylline)
The riboswitch changes conformation depending on the presence of theophylline. In the absence of theophylline, the switch prevents the binding of the mRNA to the ribosome whilst in the presence of said chemical, the conformation changes and the mRNA behind the switch can then be expressed.
Part:BBa_K411001 is an improvement of this part. We used the Part:BBa_K411003 to test the construct. [http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch#Results Experimental results] from iGEM10's NYMU-Taipei team confirm that the part works and has an increased rate of the translation of the downstream protein coding region when the concentration of Theophylline increases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]