Difference between revisions of "Part:BBa E0430"
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<partinfo>BBa_E0430 parameters</partinfo>
 
<partinfo>BBa_E0430 parameters</partinfo>
 
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<h4>Team USP-Brazil 2018 Improvement</h4>
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Team USP-Brazil, in 2018, has improved this part ([https://parts.igem.org/Part:BBa_K2771020 BBa_K2771020]) by adding it to a control gene, being that CFP constitutively expressed by ptet, which serves as a proxy for external influences on the first reporter's measurement, such as cell density, plasmid copy number and ,to some amount, metabolic burden:
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[[File:File:T--USP-Brazil--ratiometric.png|500px|thumb|none|alt=ratiometric construct|Figure 1: Scheme of USP-Brazil's improvement [https://parts.igem.org/Part:BBa_K2771020 BBa_K2771020]]]

Revision as of 19:06, 17 October 2018


EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

Standard YFP Output Device -LVA tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team USP-Brazil 2018 Improvement

Team USP-Brazil, in 2018, has improved this part (BBa_K2771020) by adding it to a control gene, being that CFP constitutively expressed by ptet, which serves as a proxy for external influences on the first reporter's measurement, such as cell density, plasmid copy number and ,to some amount, metabolic burden:

File:File:T--USP-Brazil--ratiometric.png
Figure 1: Scheme of USP-Brazil's improvement BBa_K2771020