Difference between revisions of "Part:BBa B0015"

Revision as of 20:56, 23 August 2007

double terminator (B0010-B0012)

Double terminator consisting of BBa_B0010 and BBa_B0012.
This is the most commonly used terminator. It seems to be reliable.
Note, however, that Part:BBa_B0014 is a better part for forward and reverse termination.

B0010	B0012

Secondary Structure

The mfold results are annotated with the location of the subparts BBa_B0010 and BBa_B0012 and the BioBrick assembly scar.

Using primers VR/VF2 to PCR B0010 will result in excess bands.

See a full description of the problem here.

Assembly Compatibility:

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

@@ Line 19: / Line 19: @@
 [[Image:Mfold-B0015-1.png]]
+{| width=50% style='border: 2px solid red'
+|-
+|'''Using primers VR/VF2 to PCR B0010 will result in excess bands.'''
+'''See a full description of the problem [[Problems with PCR using VR/VF2|here]].'''
+|-
+|}