Part:BBa_K812012

OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.

This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.

This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.

This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 433
  Illegal NotI site found at 287
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1648
  Illegal XhoI site found at 356
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 55
  Illegal NgoMIV site found at 164
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 79