Composite
Part:BBa_K777118
Designed by: Team Goettingen Group: iGEM12_Goettingen (2012-09-21)
motB under the control of constitutive promoter J23100
This gene codes for MotB, a part of the flagellar motor. MotA and MotB constitute the stator and are required for the flow of ions and torque generation.
Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive motB expression affect the motility of E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 351
[edit]
Categories
Parameters
//cds/membrane
//chassis/prokaryote/ecoli
//function/motility
//promoter/anderson
//chassis/prokaryote/ecoli
//function/motility
//promoter/anderson
chassis | E. coli |
function | Motility |
positive_regulators | Constitutive promoter |
protein | motB |