Protein_Domain

Part:BBa_K5466003

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-22)

Flexible linker 3x(GGGGS) Saccharomyces cerevisiae codon optimized

15 amino acid long flexible linker, designed for protein fusion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Chen, X., Zaro, J. L., & Shen, W. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357-1369. https://doi.org/10.1016/j.addr.2012.09.039

Wang, Z., Mathias, A., Stavrou, S., & Neville, D. M. (2005). A new yeast display vector permitting free scFv amino termini can augment ligand binding affinities. Protein Engineering Design And Selection, 18(7), 337-343. https://doi.org/10.1093/protein/gzi036


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/linker
Parameters
None