Part:BBa_K5223003
GjCCD4a_opt (Carotenoid cleavage dioxygenase)
Carotenoid cleavage dioxygenase is an important enzyme derived from gardenia that catalyzes the conversion of various carotenoids, including zeaxanthin, to crocin dialdehyde in vitro and in vivo. This gene is an effective tool for biotechnology production of crocin in green and non-green plant tissues. In engineered E. coli, GjCCD4a exhibits significant catalytic efficiency, with a 25.08-fold higher conversion rate than the wild-type, achieving de novo synthesis of 5 crocins from glucose, with a maximum yield of more than 10 mg/L in shake flasks[1]. In addition, when GjCCD4a was introduced into Benthamian tobacco, it led to the synthesis of crocin, in which the main active ingredients crocin I and II accounted for 99% of the total crocin[2], indicating its potential for large-scale production of these valuable compounds. The broad substrate specificity and higher efficiency of GjCCD4a make it a promising candidate for the metabolic engineering of carotenoid-derived products.
Biology and Usage
| Carotenoid cleavage dioxygenase | |
| Function | Crocin synthesis |
| Use in | Nicotiana benthamiana |
| Backbone | pZYB11-pEAQ-HT-DEST1 |
| Derived from | Gardenia jasminoides |
Reference
[1] Li S, Zhou Z, Li Y, et al. Construction of a high-efficiency GjCCD4a mutant and its application for de novo biosynthesis of five crocins in Escherichia coli. Int J Biol Macromol. 2024;277(Pt 2):133985. doi:10.1016/j.ijbiomac.2024.133985
[2] Xie L, Luo Z, Jia X, Mo C, Huang X, Suo Y, Cui S, Zang Y, Liao J, Ma X. Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana. International Journal of Molecular Sciences. 2023; 24(18):14139. https://doi.org/10.3390/ijms241814139
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |