Part:BBa_K5213000

rboh [Chondrus crispus]

Respiratory burst oxidase-like protein from Chondrus crispus;

NADPH oxidase, a membrane bound enzyme, is responsible for the generation of superoxide anions which eventually form H2O2 and other reactive oxygen species (ROS) which serve for host defense against pathogens. The oxidative burst serves as protective metabolism in red algae however its homologues have been found in higher plants, mammals as well as in Caenorhabditis elegans and Drosophila melanogaster [1]. The protein can be inhibited by diphenylene inodium (DPI), an inhibitor of flavoenzymes like NADPH oxidases.

The coding sequence of the gene contains two pairs of conserved histidine, necessary for proper NADPH oxidase activity, as a mutation in one or both causes a disruption of the electron transport chain necessary for dioxygen reduction [1][2]. Additionally a FAD and a NADPH- ribose binding site in the N-terminal region was identified, which is comparable to protein homologues from different organisms. The expression of the gene was found to be constitutive at low levels during gametophytic life cycle of the algae [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2213
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2161
  Illegal AgeI site found at 2431
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1893
  Illegal SapI site found at 1926