Part:BBa_K5038001
linker
Usage and Biology
This linker is used to link LIRA hairpin and the reporter gene. In our experiments, we use EGFP, lacZ or lacZα as the reporter gene. The presence of linker makes the LIRA system more stable i.e., more conducive to translate reporter gene and indicate the existence of target RNA.
(Figure 1. Linker has the function to link LIRA and reporter gene)
Characterization
We simulated the RNA secondary structure of LIRA-reporter system by NUPACK, using the presence or absence of the linker as a control, and judged the stability of the RNA secondary structure by its free energy, the higher the free energy, the worse the stability. The data are summarized in Figure 2.
(Figure 2. The difference of free energy without linker and free energy with linker) Temperature: 37℃
As shown in Figure 2, in most LIRA-reporter systems, the free energy in the absence of a linker is greater than the free energy in the presence of a linker, that indicates that the linker helps to enhance the stability of the LIRA-reporter system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]