Composite

Part:BBa_K4060520

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BBa_K4060513 + BBa_K4060517 + BBa_K4060504

The part that establishes the expression of BphP1, QPAS1, and HO1, and also contains a GFP reporter gene (BBa_K763004) that report the LexA signal in the cell. This part is for functional test of BphP1-LexA_QPAS1 optogenetic system. If the optogenetic system functions properly, the production of GFP should increase while under NIR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3064
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal NheI site found at 2551
    Illegal NheI site found at 2574
    Illegal NheI site found at 3965
    Illegal NheI site found at 3988
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3064
    Illegal BamHI site found at 619
    Illegal XhoI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 1853
    Illegal NgoMIV site found at 2355
    Illegal NgoMIV site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1410
    Illegal BsaI.rc site found at 2256


[edit]
Categories
Parameters
None