Composite
Part:BBa_K1520019
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
From left to right, Marker(1Kb), Pcons2-rbs-lacI-Ter, Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
Illegal BglII site found at 2222 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/biosynthesis/ahl
//function/cellsignalling
//function/cellsignalling
biology | |
control | IPTG, lacI |
direction | Forward |
ec_num | none |
function | Rep |
kegg | none |
ligands | allolactose, IPTG |
n/a | constitutive promoter family member |
negative_regulators | 1 |
o_h | |
o_l | |
positive_regulators | |
protein | luxI-LVA |
swisspro | P12747 |
tag | LVA |
uniprot | P48445 |