Coding

Part:BBa_K1071001

Designed by: Team Marburg 2013   Group: iGEM13_Marburg   (2013-09-19)

ble resistance (eucaryotic)

Zeocin (Phleomycin D1) intercalates into the DNA. It recruits copper ions as co factor and lead to a double strand break. The gene product She ble binds to Zeocin and blocks its function. It gives prokaryotes and eukaryotes a resistance against Zeocin.

Usage and Biology

This Zeocin resistance gene was used in our transfected expression vector to select transfected P. tricornutum colonies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 334
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
None