Coding

Part:BBa_I725011

Designed by: Erin Dul   Group: iGEM07_Alberta   (2007-06-18)


B-hydroxy butyryl coA dehydrogenase

B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 745
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biofuels
//function/biosynthesis/butanol
Parameters
None