Help:Submission Kit
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Requirements
Here's how part samples are sent to the Registry
Why pSB1C3?
Why do samples need to be shipped in pSB1C3?
Detailed Instructions
Instructions on how to fill out the Registry's submission form.
Submission Prep
Registry groups must follow iGEM's submission prep guidelines.
For iGEM 2015, we are standardizing the part submission process.
As in previous years, parts must be BioBrick compatible and in pSB1C3. Additionally, all part submissions must be shipped as dried DNA, in 96 well plate format, and covered with adhesive foil.
We've provided a submission kit with all of the materials you'll need to ship your parts.
- (3) 96-well skirted PCR plates
- (3) universal plate lids
- (5) adhesive foils
- bubble wrap
- a box with the iGEM HQ address, for shipping your submission
Contents
Sample Layout
Make sure to add your samples in the correct layout:
- Your first sample (s1) should be dispensed into well 1A of your submission plate.
- Your second sample (s2) should be dispensed into well 1B of the same submission plate.
Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
Sample DNA Quantity
- 250ng minimum of purified plasmid DNA (your part in pSB1C3)
- DNA must be dried down before shipping
We recommend drying down the plate in your lab's laminar flow hood.
- Sterilize the hood surface with 70% Ethanol
- Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
- Place the plate in the hood.
- With sufficient airflow, drying down 10ul (25ng/ul) of purified plasmid DNA in the plate should take four hours.
- Once all wells have dried, cover the plate with the provided adhesive foil.
Plate Labelling
Make sure to label each plate with the appropriate submission number.
Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.
Packaging your submission for shipping
Place your submission in an appropriately sized shipping container, such as a cardboard box, along with the printout of the submission form. If you use cushioning material inside the container, limit cushioning material to foam peanuts, bubble wrap, packaging paper, or similar.
Note: Part submissions packaged in materials other than the above will be rejected and these part submissions will not be taken into consideration for medals or awards. It is important that you not hide your submissions in an effort to conceal them from customs, because they will be seized.
