File:Level 2 GoldenGate.jpg
Revision as of 13:53, 13 October 2014 by Cara.deal (Talk | contribs) (Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning.)
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Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning. The product of this assembly is multiple transcriptional units within a level 2 acceptor, creating a level two, multigene construct in which neither Bsa1 nor Bpi1 restriction sites remain.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 13:53, 13 October 2014 | | 554 × 338 (44 KB) | Cara.deal (Talk | contribs) | Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning. |
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