File:Level 0 GoldenGate.jpg
Revision as of 13:52, 13 October 2014 by Cara.deal (Talk | contribs) (Level 0 parts consist of sequences with standard overhangs that have been cloned into a level 0 acceptor using the Type IIS REN BpiI. The level 0 acceptor has two inverted BpiI sites and two inverted BsaI sites. The BpiI sites are convergent on each inser)
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Level 0 parts consist of sequences with standard overhangs that have been cloned into a level 0 acceptor using the Type IIS REN BpiI. The level 0 acceptor has two inverted BpiI sites and two inverted BsaI sites. The BpiI sites are convergent on each insert and divergent on the level 0 acceptor, therefore will be removed by the process of cloning and will not exist in the assembled level 0 part. The result of the Golden Gate cloning reaction is a level 0 part that is flanked by inverted BsaI sites allowing release of the part using BsI in subsequent Golden Gate reactions.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 13:52, 13 October 2014 | | 347 × 307 (22 KB) | Cara.deal (Talk | contribs) | Level 0 parts consist of sequences with standard overhangs that have been cloned into a level 0 acceptor using the Type IIS REN BpiI. The level 0 acceptor has two inverted BpiI sites and two inverted BsaI sites. The BpiI sites are convergent on each inser |
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