Difference between revisions of "Cell-free chassis/Vesicle"
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− | + | |[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free<br>Systems (back to Intro)</font>] || <font face=georgia size=4>Introduction</font> | |
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+ | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle <font face=georgia color=#000066 size=4>Chassis<br>description</font>]</center> || | ||
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+ | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preparation_Protocol <font face=georgia color=#000066 size=4>Preparation protocol</font>]</center> || | ||
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+ | |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Vesicle/Preliminary_Testing <font face=georgia color=#000066 size=4>Preliminary Testing</font>]</center> || | ||
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Revision as of 00:13, 26 October 2007
Vesicle-Encapsulation
Homemade E. coli S30
Cell-Free Systems (back to Intro) |
Introduction |
description |
|
Description
Specifications of vesicle-encapsulation
Properties | |
Encapsulation efficiency | Measure of the amount of aqeuous contents being encapsulated as a percentage of the total amount of aqueous contents added to make the lipid-oil emulsion. |
Vesicle stability | Measure of time from vesicle-formation to the point when vesicle pops. |
Membrane permeability | Measure of the size of the largest particle that can pass through the non-specific protein pore inserted into the phospholipid bilayer. |
Method of vesicle-encapsulation
Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/CFS/Design/Protocols OpenWetWare]for the detailed protocol for forming vesicles.
References
<biblio>
- 1 pmid=12963816
- 2 pmid=16224117
</biblio>