Revision as of 22:35, 25 October 2007

Homemade E. coli S30

Cell-Free Systems (back to Intro)	Introduction
Chassis description	The Homemade E. coli S30 extract was prepared using E. coli strain BL21 (DE3) with loss of function mutations in OmpT endoproteinase and lon protease genes. This E. coli strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG 1. The simple constitutive gene expression device BBa_T9002 has been used to characterize this cell-free chassis
Preparation protocol
Preliminary Testing

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 |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 <font face=georgia color=#000066 size=4>Chassis<br>description</font>]</center> || The Homemade ''E. coli'' S30 extract was prepared using '''''E. coli'' strain BL21 (DE3)''' with loss of function mutations in OmpT endoproteinase and lon protease genes. This ''E. coli'' strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG <cite>1</cite>.
-The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_E7104 BBa_E7104] has been used to characterize this cell-free chassis
+The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_T9002 BBa_T9002] has been used to characterize this cell-free chassis
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 |<center>[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30/Preparation_Protocol <font face=georgia color=#000066 size=4>Preparation protocol</font>]</center> ||