ASU iGEM 2023

ASU iGEM 2023: Beta-FluorinX

Brief Introduction

Our Team is focused on per- and polyfluoroalkyl substances (PFAS), man-made fluorinated organic compounds whose widespread use, environmental persistence, and bioaccumulative behavior have led them to be dubbed “forever chemicals.” This project focuses on the combination of metabolic understanding as well as defluorinating enzymes to create a more robust understanding of how sustainable plastics breakdown may occur.

Compendium of Parts

On this page, you can find a compendium of various links to parts we have created in this project.

Metabolic Engineering

1. https://parts.igem.org/Part:BBa_K4895000: fadA (Salmonella Enterica)
2. https://parts.igem.org/Part:BBa_K4895001: fadB (Salmonella Enterica)
3. https://parts.igem.org/Part:BBa_K4895002: fadE (Salmonella Enterica)
4. https://parts.igem.org/Part:BBa_K4895004: fadBAE Polycistronic expression of all three enzymes

Defluorinating Constructs

1. https://parts.igem.org/Part:BBa_K4895051: DeHa1 (Delftia Acidovorans)
2. https://parts.igem.org/Part:BBa_K4895052#DeHa2_.28Delftia_Acidovorans.29: DeHa2 (Delftia Acidovorans)
3. https://parts.igem.org/Part:BBa_K4895056: DEH1 Moraxella sp. B
4. https://parts.igem.org/Part:BBa_K4895057: FACD Burkholderia
5. https://parts.igem.org/Part:BBa_K4895058: FACD Rhodopseudomonas

MiKOP

1. https://parts.igem.org/Part:BBa_K4895995: MiKOP Composite part
2. https://parts.igem.org/Part:BBa_K4895996: MiKOP Insert 1
3. https://parts.igem.org/Part:BBa_K4895997: MiKOP Insert 2