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  • 12:28, 9 September 2015 Saras20 (Talk | contribs) uploaded File:Cas3 1.png (Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)