Project

Part:BBa_K404124:Design

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-10-11)
Revision as of 23:14, 27 October 2010 by Bea (Talk | contribs) (→‎Source)

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[AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1122
    Illegal NgoMIV site found at 1780
    Illegal NgoMIV site found at 2685
    Illegal AgeI site found at 2814
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3216
    Illegal SapI.rc site found at 1172


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of two parts was conducted following the proposed BioBrick Assembly Strategy (https://parts.igem.org/Assembly:Standard_assembly). Since the gene of interest was designed in the RFC25 standard, BioBrick assembly leads to a scar and the additional restriction enzymes NgoMIV and AgeI.

Source

mGMK_TK30: Herpes simplex virus
phTERT : Human telomerase promoter
Misc: pAAV_MCS (Stratagene)

References

Reference

Cech, T., 2000. Life at the End of the Chromosome: Telomeres and Telomerase. Angewandte Chemie (International ed. in English), 39(1), 34-43. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10649348.

Kyo, S. et al., 2008. Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers. Cancer science, 99(8), 1528-38. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18754863.

Wick, M., Zubov, D. & Hagen, G., 1999. Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT). Gene, 232(1), 97-106. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10333526.