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Part:BBa_K331030:Experience

Designed by: Adam Smith   Group: iGEM10_Lethbridge   (2010-10-07)
Revision as of 20:09, 27 October 2010 by Liszabruder (Talk | contribs) (→‎Applications of BBa_K331030)

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Applications of BBa_K331030

In order to further characterize the C-terminal and N-terminal oligoarginine tag (BioBricks BBa_K249005 and BBa_K249004 respectively) and investigate the effect their placement on protein stability, yellow fluorescent proteins (YFP) with the oligoarginine fused to either the C-terminus (BBa_K331023) or N-terminus (BBa_K331022) (and preceded by a ribosomal binding site – BBa_B0034) were synthesized. We used our Red/White 3-Antibiotic assembly method to add a tetracycline repressible promoter (BBa_R0040) for constitutive expression of the fusion protein. This addition generated BioBricks BBa_K331031 and BBa_K331030 for the C-terminal tagged and N-terminal tagged YFP respectively.

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