Regulatory

Part:BBa_K395008

Designed by: Eriko Uchikoshi   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 17:59, 27 October 2010 by Kitano (Talk | contribs)

Plux (rep) R0061middle

R0061 is the promoter repressed by LuxR in the presence of AHL. We designed this promoter by changing one base of the existing BioBrick (BBa_R0061).

Tokyotech R0061 K395008 graph K395008.jpg

In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1. In order to measure activity of R0061, we introduced K395105 and S03119 (pTetR-rbs-luxR-ter) on pSB3K3. The left figure shows that this promoter is repressed by 3OC6HSL and the strength of this promoter is weaker than R0061.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep#R1, characterization of R0061 & K395008 (promoter repressed by LuxR/3OC6HSL) more information])














Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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