Reporter

Part:BBa_K395105:Design

Designed by: Shohei Kitano   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 17:49, 27 October 2010 by Kitano (Talk | contribs) (→‎Design Notes)

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GFP reporter repressed by LuxR and 3OC6HSL (K395008:K121013)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal BamHI site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 756


Design Notes

Source

We ligated BBa_K395008 into BBa_K121013.

References

Conversion of the Vibrio fischeri Transcriptional activator, LuxR, to a repressor: Egland, Kristi A. and Greenberg, E. P. Journal of Bacteriology. Feb 2000, p. 805-811: http://jb.asm.org/cgi/content/abstract/182/3/805