Plasmid

Part:BBa_K422015:Design

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-26)
Revision as of 10:13, 27 October 2010 by Zwickyk (Talk | contribs) (→‎References)

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pSEVA132


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 292
    Illegal EcoRI site found at 1505
    Illegal XbaI site found at 3478
    Illegal SpeI site found at 1
    Illegal SpeI site found at 3127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 292
    Illegal EcoRI site found at 1505
    Illegal SpeI site found at 1
    Illegal SpeI site found at 3127
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 292
    Illegal EcoRI site found at 1505
    Illegal BglII site found at 1899
    Illegal BamHI site found at 1464
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 292
    Illegal EcoRI site found at 1505
    Illegal XbaI site found at 3478
    Illegal SpeI site found at 1
    Illegal SpeI site found at 3127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 292
    Illegal EcoRI site found at 1505
    Illegal XbaI site found at 3478
    Illegal SpeI site found at 1
    Illegal SpeI site found at 3127
    Illegal NgoMIV site found at 5397
    Illegal NgoMIV site found at 5891
    Illegal NgoMIV site found at 6020
    Illegal AgeI site found at 1299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1597
    Illegal SapI site found at 1281
    Illegal SapI site found at 3733
    Illegal SapI.rc site found at 3061
    Illegal SapI.rc site found at 3409


Source

Victor de Lorenzo's lab

References

Victor de Lorenzo's lab

http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning