Regulatory

Part:BBa_K395008

Designed by: Eriko Uchikoshi   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 04:02, 27 October 2010 by Kitano (Talk | contribs)

Plux (rep) R0061middle

We designed a new promoter which is repressed by AHL and LuxR complex by changing one base of the existing BioBrick parts (BBa_R0061).

Tokyotech R0061 K395008 graph K395008.jpg

In order to characterize this promoter, we constructed K395105 combining K395008 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) and this backbone is pSB6A1. We used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control.














Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None