Device

Part:BBa_K337021

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-21)
Revision as of 02:39, 27 October 2010 by Dniopek (Talk | contribs)

BGH(rc)/shRNA10(rc)/sv40(rc)/CMV(fw)

SV40 promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator (reverse complementary). The cDNA of the users' choice can be introduced behind the RSV promoter. (BB-2, RFC12)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None