Device

Part:BBa_K299817

Designed by: Michał Lower   Group: iGEM10_Warsaw   (2010-10-13)
Revision as of 23:20, 26 October 2010 by Joanna (Talk | contribs)

This part is licensed under
Creative BioCommons

control construct - GFP under J23102


Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellulat protein targeting. Originally from Aequorea victoria.

Authors:

Cloned by Joanna Leszczyńska.

Work supervised by Michał Lower.

Construct design

The part consists of J23102 promoter and B0032 RBS ligated to inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). GFP as marker. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 708


[edit]
Categories
Parameters
n/acontrol construct - GFP under J23102