Project

Part:BBa_K404229

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 12:26, 26 October 2010 by Achim (Talk | contribs)

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP)
The Biotinylation Acceptor peptide motif, including a knockout of the natural HSPG tropism, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587KO-BAP.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4197
    Illegal BsaI site found at 4379
    Illegal BsaI site found at 4416
    Illegal SapI site found at 3048


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/capsid_coding
Parameters
None