Project

Part:BBa_K404167

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-10-24)
Revision as of 11:49, 26 October 2010 by Achim (Talk | contribs)

pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)

<b>Construct containing the mVenus N-terminal fusion to VP2, a NLS & VP1up insertion and the Biotinylation Acceptor peptide motif in the 587 loop</b>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3149
    Illegal XhoI site found at 698
    Illegal XhoI site found at 884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3735
    Illegal SapI site found at 2586


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors/aav
//viral_vectors/aav/capsid_coding
Parameters
None