Reporter

Part:BBa_K422014

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)
Revision as of 10:36, 26 October 2010 by Zwickyk (Talk | contribs) (→‎Cloning strategy)

mCyPet (AarI A-part)

Biological Background

CyPet is an evolutionary optimized cyan fluorescent protein CFP developed in 2005 by Nguyen and Daugherty. They showed the FRET pair CyPet-YPet to have a 7-fold higher ratiometric FRET signal change than their parental pair.

mCyPet has a mutation in A206K preventing it from forming dimers (Ohashi et al., 20007).

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mcyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 733
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 733
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 733
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 733
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


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