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Part:BBa_K337019:Design

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-21)
Revision as of 08:56, 26 October 2010 by Dniopek (Talk | contribs) (→‎Source)

FRT site with spacing


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 155
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 155
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 155
    Illegal NgoMIV site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

as the FRT site is a pretty short sequence we added some spacing in front and behind to enable easy cloning of this part.


Source

pcDNA5/FRT/TO (Invitrogen)

References