Regulatory

Part:BBa_K302031:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 01:29, 26 October 2010 by PhilipHall (Talk | contribs)

Sucrose sensitive inducer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence selected based on the work of Steinmetz et al. and Meng and Futterer in the papers below

Source

Bacillus subtilis 168 strain

References

Steinmetz, M., Le Coq, D., Aymerich, S., Gonzy-Treboul, G. & Gay, P. (1985). The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol Gen Genet 200, 220–228.

Meng, G. & Futterer, K. (2003). Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat Struct Biol 10, 935–941.