Generator

Part:BBa_K299505

Designed by: Michał Lower   Group: iGEM10_Warsaw   (2010-08-20)
Revision as of 23:31, 25 October 2010 by Mlower (Talk | contribs)

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Expression Vector pT7+B0030

This part is licensed under
Creative BioCommons

This is expression vector containing B0030 under T7 promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.

Authors

Clonning:

  • Ania Puławska
  • Milena Bażlekowa

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Construct design

The device consists of T7 promoter followed by B0030 RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.

Expression strength

Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.

It's estimated to be 91,84% relative to BBa_K299509, the strongest ExpressIt! vector.

Summary for the whole ExpressIt! family is shown below:

ExpressIt.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aExpression Vector pT7+B0030