Measurement

Part:BBa_K299028

Designed by: Anna Olchowik   Group: iGEM10_Warsaw   (2010-10-16)
Revision as of 22:52, 25 October 2010 by Mlower (Talk | contribs)

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RBS measurement device pT7 J61117 GFP

This part is licensed under
Creative BioCommons

We planned to use this device to determine BBa_J61117 strength relative to BBa_B0034 using cell-free system.

Authors

Clonning:

  • Ania Puławska
  • Milena Bażlekowa

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Construct design

The device consists of BBa_I719005 T7 promoter followed by BBa_J61117 Anderson's RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein. Device was created by cloning BBa_I13401 behind ExpressIt! vector containing appropriate RBS.

In this case physical DNA after J61117 is suitable to create biobrick scars in the composite parts, therefore we could use the standard biobrick assembly option in the registry (default scars).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


[edit]
Categories
Parameters
n/aRBS measurement device pT7 J61117 GFP