Measurement

Part:BBa_K299020

Designed by: Anna Olchowik   Group: iGEM10_Warsaw   (2010-10-16)
Revision as of 22:46, 25 October 2010 by Mlower (Talk | contribs)

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RBS measurement device pT7 B0030 GFP

This part is licensed under
Creative BioCommons

We planned to use this device to determine BBa_B0030 strength relative to BBa_B0034 using cell-free system.

Authors

Clonning:

  • Ania Puławska
  • Milena Bażlekowa

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Construct design

The device consists of BBa_I719005 T7 promoter followed by BBa_B0030 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein. Device was created by cloning BBa_I13401 behind ExpressIt! vector containing appropriate RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


[edit]
Categories
Parameters
n/aRBS measurement device pT7 B0030 GFP