Device
Part:BBa_K302012:Experience
Designed by: Rachel May Boyd, Phil Hall Group: iGEM10_Newcastle (2010-08-12)
Revision as of 22:37, 25 October 2010 by RachelBoyd (Talk | contribs) (→Applications of BBa_K302012)
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Applications of BBa_K302012
The filamentous cell part is repressed by LacI . The LacI repression is removed by induction with IPTG. Without LacI repression a higher proportion of the cells are filamentous. The filamentous cell phenotype is achieved by overexpressing yneA which is an indirect inhibitor of cell division.
| Graph1: |
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| Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168. |
| See below: Bacillus subtilis 168 cells (left),Bacillus subtilis expressing yneA(centre) and Bacillus subtilis overexpressing yneA(right) |
  
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| Graph2: |
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| Graph 2 shows the percentage of cells at different lengths(μm)uninduced |
| See below: Bacillus subtilis 168 cells (left) and non-induced cells(right) |
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| Graph3: |
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| Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right) |
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| Graph4: |
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| Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right) |
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Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.
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