Measurement

Part:BBa_K299008

Designed by: Anna Olchowik   Group: iGEM10_Warsaw   (2010-07-19)
Revision as of 22:35, 25 October 2010 by Mlower (Talk | contribs)

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RBS measurement device J23100 B0033 GFP

This part is licensed under
Creative BioCommons

This device was used to determine BBa_B0033 strength relative to BBa_B0034.

Authors

Clonning:

  • Ania Puławska
  • Ania Safray
  • Milena Bażlekowa

The lab work was supervised by:

  • Jarek Pankowski
  • Ania Olchowik
  • Kasia Grześ

Flow cytometer measurements done by Cherry Moreno

Construct design

The device consists of BBa_J23100 promoter followed by BBa_B0033 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein.

Measurements

Using this construct we have determined that BBa_B0033 strength is 0.35% relative to BBa_B0034

All our measurement results are summarized on the chart below:

RBS_relative_strength.png

For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704


[edit]
Categories
Parameters
n/aRBS measurement device J23100 B0033 GFP