Coding

Part:BBa_K302029:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-04)
Revision as of 16:52, 25 October 2010 by PhilipHall (Talk | contribs) (→‎Design Notes)

sacB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sacB sequence encodes for levansucrase enzyme of B. Subtilis. The gene has been modified by removing an ecoRI at position 201 using B. Subtilis codon bias and by changing the stop codon for the recommended double TAA.

Source

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References