Coding

Part:BBa_K422011:Design

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)
Revision as of 08:52, 25 October 2010 by Zwickyk (Talk | contribs) (→‎Design Notes)

mreB (AarI C-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 170
    Illegal PstI site found at 790
    Illegal AgeI site found at 487
    Illegal AgeI site found at 805
    Illegal AgeI site found at 892
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. MreB-C is an C-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Escherichia coli genome

References

http://2010.igem.org/Team:ETHZ_Basel

Kruse, Bork-Jensen and Gerdes: The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex. Molecular Microbiology. 2005;55:1.