Part:BBa_K325909

Input: L-Arabinose Output: Light

pBad/araC I0500 PoPS to Light

---

Description
G28 !

THIS PAGE IS CURRENTLY BEING UPDATED.

Performance

Experiment1	Characteristic1	Value1
Transfer Function	Maximum Output	6.6 PoPS cell-1
	Hill coefficient	1.6
	Switch Point	1.5E-9 M 3OC6HSL, exogenous
Response time:	<1 min
Input compatibility	Strong response to	3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
	Weak response to	C4HSL, C10HSL, C12HSL
Stability	Genetic Stability (Low/High Input)	>92/>56 generations
	Performance Stability (Low/High Input)	>92/>56 generations
Demand	Internal Demand (Low/High Input)	Not measured
	Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides	(0/6xNt) nucleotides cell-1 s-1
		(0/1.5E-1xNt) RNAP cell-1
	Growth Rate (Low/High Input)	54/59 min Doubling time

¹Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176.

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.