Part:BBa_K314203

HSL inducible toxin antitoxin, Tse2 + Tsi2
This part contains the Tse2/Tsi2 locus from Pseudomonas aeruginosa downstream from an HSL inducible promoter(F2620)

Usage and Biology

This composite part produces the toxin Tse2 and its antitoxin Tsi2 when cells are exposed to HSL. Tse2 is a potent cytotoxin. Tsi2 is its antitoxin, which protects the host cell from death by Tse2. Tse2 is targeted for export from the host cell cytoplasm into the cytoplasm of Gram-negative bacteria by the Type VI secretion system, killing the target Gram-negative cells unless they also express Tsi2. Therefore, this part is useful for regulating secretion of Tse2.

Functional Parameters

This part has been tested in pSB3K3. E. coli transformed with this part showed Tse2 production in a broth culture containing HSL at a concentration of 10µM, while no appreciable level of Tse2 was produced in an environment not containing HSL. This was verified by an anti-Tse2 western blot of cellular supernatant of colonies grown up in liquid cultures containing either 10µM HSL or no HSL for a period of approximatively 24 hours.

E. coli showed Tse2 production in response to HSL

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1283
  Illegal NgoMIV site found at 1340
  Illegal NgoMIV site found at 1465
  Illegal NgoMIV site found at 1795
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1004
  Illegal SapI.rc site found at 1790