Measurement

Part:BBa_K300091

Designed by: Giacomo Zambianchi, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-19)
Revision as of 13:09, 19 October 2010 by 110hs (Talk | contribs)

3OC6HSL inducible generator for BBa_K300095

This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasin (BBa_K300002), intein (BBa_K300004), linkers (BBa_K105012) and GFP reporter (BBa_K300005). With this BioBrick was also tested the right folding of the recombinant proteins, but at the same time it can also be considered a device for purification of these proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2859
    Illegal SapI.rc site found at 2165


[edit]
Categories
Parameters
None