Reporter
Part:BBa_K389402
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-10-11)
Sensitivity tuner + luciferase
This is a modified BBa_I746380 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1295
Illegal NgoMIV site found at 2639
Illegal NgoMIV site found at 2660
Illegal AgeI site found at 818
Illegal AgeI site found at 930
Illegal AgeI site found at 1132
Illegal AgeI site found at 2363 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2545
[edit]
Categories
Parameters
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
None |