Plasmid_Backbone

Part:BBa_K300001:Design

Designed by: Susanna Zucca, Lorenzo Pasotti, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)
Revision as of 16:11, 3 October 2010 by Registry (Talk | contribs)

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BioBrick integrative base vector for S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal XbaI site found at 46
    Illegal SpeI site found at 2
    Illegal PstI site found at 261
    Illegal PstI site found at 1607
    Illegal PstI site found at 3663
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3727
    Illegal NheI site found at 1549
    Illegal NheI site found at 1600
    Illegal SpeI site found at 2
    Illegal PstI site found at 261
    Illegal PstI site found at 1607
    Illegal PstI site found at 3663
    Illegal NotI site found at 3733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3727
    Illegal BglII site found at 51
    Illegal XhoI site found at 1499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3727
    Plasmid lacks a suffix.
    Illegal XbaI site found at 46
    Illegal SpeI site found at 2
    Illegal PstI site found at 261
    Illegal PstI site found at 1607
    Illegal PstI site found at 3663
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3727
    Plasmid lacks a suffix.
    Illegal XbaI site found at 46
    Illegal XbaI site found at 3742
    Illegal SpeI site found at 2
    Illegal PstI site found at 261
    Illegal PstI site found at 1607
    Illegal PstI site found at 3663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2695


Design Notes

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Source

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References