Coding

Part:BBa_K322237

Designed by: Chris French and William Galdric Rostain   Group: iGEM10_Edinburgh   (2010-09-26)
Revision as of 10:55, 26 September 2010 by Registry (Talk | contribs)

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Firefly luciferase from Photinus pyralis

In luminescent reactions, light is produced by the oxidation of a luciferin (a pigment): luciferin + O2 → oxyluciferin + light

Thus luciferase is a a reporter protein when connected to other proteins or promoters prodicing light, by the oxidation of substrate luciferin.

This is derived from BioBrick I712019, but PCR was used to remove the extra bases before the start codon (these are shown in the Registry sequencing data but are not indicated in the sequence as entered by Ljubljana 2007; presumably they are there to make a proper eukaryotic context for the start codon). This BioBrick contains the bare coding sequence in pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


[edit]
Categories
//cds/reporter
//function/reporter/light
Parameters
emission555 nm