Plasmid_Backbone
Screening

Part:pSB1A10:Design

Designed by: Josh Michener, Jason Kelly   Group: iGEM06_MIT   (2006-05-26)
Revision as of 08:00, 26 May 2006 by Jasonk (Talk | contribs)

The process for building pSB1A10,was:

  1. Build I13534 using normal BB construcion.
  2. Insert I13534 into psb1A3 plasmid
  3. Scar out the BB prefix and suffix using Part:BBa_I13450
  4. Re-insert a BB prefix/suffix into the center of I13534 using primers to the "insertion region" I13457 of and around-the-horn PCR.

Some design notes:

  1. The inclusion of the 'barcode' was unintentional (it was attached to Part:BBa_E1010), it has no (intended) function.
  2. The "spacer artifacts" at position 22 and 5225 are artifacts from around-the-horn PCR for inserting the BB restriction sites into the center of the plasmid. If you look at Part:BBa_I13457 you will see the corresponding regions in the section annotated "insertion region".