User:VolkerMorath
Virus Construction Kit
info AT biobricks DOT org
Viral Vectors are modified viruses that are used in molecular biology to transfer genetic material into desired cells. The adventage of viral vectors is that the different viral mechanismns can be employed to transduce the target cell in an specific and efficient manner. This process is called transduction when eucaryotic cells are the target of the gene transfer.
Key properties of a viral vector
Viral vectors are tailored to their specific applications but generally share a few key properties.
- Safety: Viral vectors are often engineered on the basis of an human infectious virus. To reduce the risks for people that handle the virus and patient of an viral vector base therapy the genetic material of the viurs is manipulated in a way to knock-out the ability of the viral vector to reproduce. To reach this requirement the genes coding for the production of the viral capsid are often provided in trans to ensure that the viral vector does not contain the information for
- Low toxicity:
- Stability:
- Cell type specificity:
- Identification:
Double stranded DNA Viruses
The Adenovirus
| [http://expasy.org/viralzone/all_by_species/183.html Adenovirus] | |
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| Group: | ds DNA Virus |
| Family: | Adenoviridae |
| Genus: | Mastadenovirus |
| Host range | Human, mammals |
| Capacity: | 35000bp |
The Simian virus 40
| [] | |
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| Group: | ds DNA Virus |
| Family: | |
| Genus: | |
| Host range | |
| Capacity: | |
Single stranded DNA Viruses
The Adeno-associated Virus
| [http://expasy.org/viralzone/all_by_species/226.html Adeno-associated Virus] | |
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| Group: | ss DNA Virus |
| Family: | Parvoviridae |
| Genus: | Dependovirus |
| Host range | Human, vertebrates |
| Capacity: | 4700bp |
In the iGEM competition 2010 the Team Freiburg bioware constructed a Virus Construction Kit that makes the Adeno-associated virus (AAV2) available for all users of the parts registry.
The Adeno-associated virus is a well studied vector for eukaryotic gene transfer.
Double stranded RNA Viruses
Single stranded RNA (+) Viruses
Single stranded RNA (-) Viruses
Double stranded RNA retro-transcribing Viruses
Single stranded RNA retro-transcribing Viruses
The Lentivirus
| [http://expasy.org/viralzone/all_by_species/264.html Lentivirus] | |
|---|---|
| 200px | |
| Group: | ds DNA Virus |
| Family: | Retroviridae |
| Genus: | Lentivirus |
| Host range | Vertebrate |
| Capacity: | 9750bp |
References: 1)http://expasy.org/viralzone/
