Composite
Part:BBa_K389013:Design
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-09-11)
VirA reporter system mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1686
Illegal AgeI site found at 1798 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
medium strong constitutive promotor, terminator to keep basal expression of mRFP low
Source
parts.igem, synthetic gene by Mr. Gene, TI-plasmid from Agrobacterium tumefaciens C58